The purpose of this Funding Opportunity Announcement (FOA) is to provide regional access for cryoelectron microscopy (cryoEM) laboratories to state-of-the-art data collection capabilities. NIGMS will support consortia of established and early stage investigator laboratories whose research has an established specialization in and dependence on cryoEM. These laboratories will coordinate with each other to share facilities and resources for direct electron detection. Consortia will consist of a host institution which already has a modern high-performing cryoEM installation and proven capabilities for high-resolution data collection, partnered with regional participating institutions. A consortium will support surplus capacity for cryoEM data collection (infrastructure and services) at the host institution and make it available as a resource for the participating regional institutions. Projects will support access for cryoEM laboratories at participating institutions to resources and services at host sites. Projects will (a) contribute to the fixed costs of maintaining the host facility and extend services in proportion to the resources dedicated to the regional users and (b) support access to the facility by the regional laboratories. The host institution need not already have an electron detector; this FOA can help fund acquisition of direct detection equipment. Support is limited to enabling the participating cryoEM laboratories to collect data and perform the initial stages of processing raw images, and does not include other research activities. Consortia will consist of a minimum of 3-5 institutions; award budgets will depend on the number and data collection needs of the regional participating cryoEM laboratories. This initiative is not intended to support service centers of the traditional type in which the host laboratory performs the cryoEM analysis for non-specialist collaborators.