This funding announcement is a Notice of Intent to Award (NOI): PLEASE DO NOT APPLY FOR THIS OPPORTUNITY. Federal regulations require that all funding opportunities be posted on Grants.gov. This posting is intended to comply with those regulations. This is a single source opportunity. Summary: This study seeks to compare CTS larval density in natural and created pools of varying age using quantitative enclosure sampling, a standardized technique. Also, using densities and pool acreages the true number of larvae in pools will be estimated, which can be used to calibrate the accuracy of timed dipnet surveys. Although only pitfall trapping can definitively determine successful recruitment, the likelihood of recruitment will be better understood building upon two years of hydroperiod and larval growth rate data. The 11-year study of larval abundance and pool features (area, maximum depth, etc) conducted by David Cook will be used as baseline data in this study. In general, the data will provide preserve managers with information about the performance of created pools compared with natural pools. The information will also be used as the basis for future studies on pool parameters that lead to successful reproduction and recruitment, which can then be incorporated into pool design criteria. The proposed study would include 8 natural and 8 created pools of varying ages (1-3 yrs, 4-10 yrs, and > 20 yrs) located at 3-4 preserves located on the Santa Rosa Plain and owned by CDFG.